Electrocatalytic amplification of DNA-modified nanoparticle collisions via enzymatic digestion† †Electronic supplementary information (ESI) available: Supporting data including size distribution analysis of the PtNPs by TEM, a fluorescence calibration curve for estimating the coverage of ssDNA on PtNPs, ECA of PtNP@ssDNA modified with thiol on the 3′-end, and i–t curves for collisions of naked PtNPs at Au UMEs. See DOI: 10.1039/c6sc02165d Click here for additional data file.

Electrocatalytic amplification of DNA-modified nanoparticle collisions via enzymatic digestion.

We report a new and general approach that will be useful for adapting the method of electrocatalytic amplification (ECA) to biosensing applications. In ECA, individual collisions of catalytic nanoparticles with a noncatalytic electrode surface lead to bursts of current. In the work described here, the current arises from catalytic electrooxidation of N2H4 at the surface of platinum nanoparticle...

Single nanoparticle collisions at microfluidic microband electrodes: the effect of electrode material and mass transfer.

We report on the effect of convection on electrochemically active collisions between individual Pt nanoparticles (PtNPs) and Hg and Au electrodes. Compared to standard electrochemical cells utilizing Hg and Au ultramicroelectrodes (UMEs) used in previous studies of electrocatalytic amplification, microelectrochemical devices offer two major advantages. First, the PtNP limit of detection (0.084 ...

Nanomechanical properties of protein–DNA layers with different oligonucleotide tethers† †Electronic supplementary information (ESI) available: The different presentation of QCM-D readout is shown and the analysis of kinetics is presented. See DOI: 10.1039/c6ra10090b Click here for additional data file.

Table of contents page Frequency f n /n and bandwidth Γ n /n shifts belonging to the QCM-D measurements presented in Figures 1-4, and Figure 6 in the main manuscript. S2 Analysis of the binding and unbinding kinetics of a Cy5-modified flavin-dsDNA and a Cy5-modified flavin-ssDNA ligand measured by SPFS. S5 Analysis of the binding and unbinding kinetics of a Cy5-modified flavin-dsDNA and a Cy5-m...

Real-Time PCR: an Appropriate Approach to Confi rm ssDNA Generation from PCR Product in SELEX Process

Background: Aptamers are single stranded DNA (ssDNA) or RNA molecules. The potential of aptamers for binding to the different targets has made them be widely used as the preferred diagnostic and therapeutic tools. DNA aptamers present several advantages over the RNA oligonucleotides due to their higher stability, easier selection, and production. Selection of DNA aptamers which...

Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling† †Electronic supplementary information (ESI) available: Synthetic procedures for the building blocks and DNA strands, full spectroscopic analysis of the ssDNA and duplex systems. See DOI: 10.1039/c5ob01681a Click here for additional data file.